Figure 10.

Expression of p73 in E2F1 knock-out mice. A, C, Immunoblot analysis of p73 in cortical tissue extracts probed with N-terminal (A) and C-terminal (C) antibodies. Note the important reduction of TAp73 levels at 82 kDa (TAp73α) and at 72 kDa (probably representing TAp73β) in mutant mice compared with the controls. After incubation with a C-terminal antibody, a DNp73 isoform at 66 kDa shows only a slight reduction in the E2F1 mutant (C). B, D, α-Tubulin controls to test the amount of protein analyzed.