Figure 1.
LTDi is mediated by endocannabinoids and is independent of postsynaptic Ca2+ influx. A, LFSi of afferents in the LA (100 pulses/1 Hz) leads to a significant long-term depression of IPSCs recorded in principal BLA neurons (LTDi) to 78 ± 5% of control (n = 6; p < 0.05). B, Pretreatment of the slices with the CB1 receptor agonist WIN55,212-2 (5 μm) occludes the induction of LTDi (104 ± 10% of control; n = 5; p > 0.05). LTDi without WIN55,212-2 (data from A) is shown for comparison (squares). C, LTDi is not inhibited when BAPTA (40 mm) is applied to the postsynaptic cell (67 ± 7% of control; n = 7; p < 0.05). Representative traces of IPSCs before (1) and after (2) LFSi are shown. The experiments were performed in C57BL/6JOlaHsd mice.