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. 2019 Aug 30;10:1965. doi: 10.3389/fmicb.2019.01965

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Copyright © 2019 Navarro-Garcia, Ruiz-Perez, Cataldi and Larzábal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Model of T6SS baseplate assembly. (A) Schematic representation of the protein-protein interaction in the assembly of the TssKFGE wedge complex. The wedge complex is composed of five essential subunits: TssK, TssF, TssG, TssE with a stoichiometry of 6 (two trimers), 2, 1 and 1 subunits, respectively (theoretical mass, 498 KDa). According to the Cryo-EM density map, two wing-like structures (TssF) are wrapping a central backbone (TssG and probably TssE) and a root-like structure (TssK). Biogenesis of the baseplate occurs by the polymerization of six wedges around the central VgrG. (B) Schematic representation of the baseplate assembly complex (top, bottom and profile view).