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. 2004 Nov 17;24(46):10511–10520. doi: 10.1523/JNEUROSCI.2828-04.2004

Figure 4.

Figure 4.

CST fibers in the caudal spinal cord of NgR (310)ecto-Fc-treated rats. A, B, Parasagittal sections of the distal spinal cord from different NgR(310)-Fc-treated rats show CST fibers with a highly branched pattern. C, Parasagittal section of the distal spinal cord from an NEP1-40-treated rat illustrates regenerating fibers with a lesser degree of branch complexity. D, CST fiber number at various distances caudal to the injury site from NgR(310)ecto-Fc (n = 6 rats) and control (Ctrl; n = 6 rats) animals is reported. E, Counts of fibers (number of fibers ≥200 μm in length per rat) outside of the vCST from sagittal sections 1-10 mm caudal to SCI show a greater number in the NgR(310)ecto-Fc-treated (n = 6 rats) and NEP1-40-treated groups (n = 7 rats) than in the control group (n = 6 rats). F, CST sprout complexity (total length of fiber arbor per sprout as visualized in a single section) is quantitated for vehicle-treated (n = 7 rats), NgR(310)ecto-Fc-treated (n = 7 rats), and NEP1-40-treated (n = 7 rats) rats. The arbor length per sprout in both sets of NgR antagonist-treated rats is much greater than control rats. The difference between NgR(310)-Fc and NEP1-40 is significant (p < 0.01; Student's t test). G, H, Transverse section at a level 11-15 mm caudal to the lesion from an NgR(310)ecto-Fc-treated rat illustrates a number of CST fibers with branching patterns in caudal spinal cord gray matter (G) and white matter (H). I, Quantification of CST fibers from transverse sections 11-15 mm distal to the lesion indicates a greater number BDA-labeled fibers in NgR(310)ecto-Fc-treated (n = 6 rats) and NEP1-40-treated (n = 7 rats) animals than the in control group (n = 6). Scale bar: A-C, G, H, 25 μm. Means + SEM in bar graphs are reported. The values from the NgR(310)ecto-Fc- or NEP1-40-treated group are statistically different from the control group (D, E, I) (*p < 0.05; **p < 0.01; Student's t test).