Figure 1.

a, Immunoblots of mGlu4 receptors in rat cerebellar cultures plated in CDM (lane 1), BME (lane 2), and BME plus 10% FCS (lane 3). The adult rat cerebellum (lane 4) is shown as a positive control. Immunoreactive bands corresponding to monomeric mGlu4 receptor are at ∼100 kDa. These bands were absent in the cerebellum of mGlu4 knock-out mice (data not shown). b, Concentration-dependent inhibition of [³H]thymidine incorporation by PHCCC (24 hr of exposure) in cultures of rat cerebellar granule cells. Values are means ± SEM of six determinations from two independent experiments. c, PHCCC reduced the number of BrdU⁺ GNPs in rat cerebellar cultures. BrdU⁺ cells were counted in four microscopic fields from 12 culture dishes from three independent experiments by an observer who was unaware of the treatment. Values are means ± SEM. p < 0.05 (1-way ANOVA plus Fisher's PLSD) versus the corresponding values in the absence of PHCCC (^*) or versus PHCCC alone (#).