Figure 3.

a, RT-PCR analysis of mGlu4 receptor mRNA in PC12 and U87MG cells; the rat cerebellum is shown as a positive control. b, Effect of a 24 hr exposure to PHCCC on [³H]thymidine incorporation in PC12 cells. Values are means ± SEM of 12-15 determinations from four to five independent experiments. ^*p < 0.05 (Student's t test) compared with control (CNT) cultures. c, Effect of a 24 hr exposure to PHCCC on [³H]thymidine incorporation in cultured U87MG cells. Values are means + SEM of six determinations from two independent experiments. d, Immunoblot of mGlu4 receptors in cultured HEK 293 cells transfected with the empty vector (mock) or with mGlu4 receptor cDNA. e, Effect of a 24 hr exposure to PHCCC on [³H]thymidine incorporation in mock or mGlu4 receptor expressing HEK 293 cells. Values are means + SEM of six determinations from two independent experiments. ^*p < 0.05 (Student's t test) compared with control (Ctrl) cultures.