Figure 4.

A 24 hr exposure to PHCCC promotes neurite growth in cultured granule cells. Phase-contrast microphotographs of control cultures and cultures treated with 30 μm PHCCC are shown in a and b. The number of neurites of different length per microscopic field in control cultures and in cultures treated with PHCCC or l-AP-4 (both at 30 μm) is reported in c; the number of treated cells bearing neurites of different length is shown in d. Values are means ± SEM of six determinations from two independent experiment. Each value is the mean of determinations obtained from four microscopic fields/dish by an observer who was unaware of the treatment. ^*p < 0.05 (1-way ANOVA plus Fisher's PLSD) versus the respective controls. Scale bar, 20 μm.