Figure 5.

Photomicrographs of cultured granule cells plated in BME and stained for the neuronal marker TUJ1 are shown in a and b. Control cultures are shown in a, and cultures treated with 30 μm PHCCC for 24 hr are shown in b. In c, TUJ1-positive cells were counted from five randomly selected microscopic fields per dish by an observer who was unaware of the treatment. Values (means ± SEM) were calculated from six culture dishes per group. ^*p < 0.05 (Student's t test) compared with control cultures. Scale bar, 50 μm. The total cell number in five microscopic fields was 510 + 36 and 465 + 21 (n = 6 culture dishes) in control and PHCCC-treated cultures, respectively. The total number of TUJ1-positive cells was 270 + 18 and 339 + 22 in control and PHCCC-treated cultures, respectively.