Table 2.
Inhibition of [3H]thymidine incorporation in cultured cerebellar granule cells grown for 24 hr under different conditions
|
|
[3H]thymidine incorporation (dpm/dish) × 10−3 |
||||
|---|---|---|---|---|---|
|
|
BME |
BME plus 10% FCS |
CDM |
||
| Control | 482 ± 18 | 864 ± 5 | 1324 ± 16 | ||
| PHCCC (30 μm) | 194 ± 36a | 508 ± 19a | 416 ± 16a | ||
| Insulin (0.5 mg/ml) | 756 ± 4 | 1190 ± 7 | 1588 ± 73 | ||
| Insulin plus PHCCC | 466 ± 44a,b | 588 ± 14a | 418 ± 20a | ||
| SHH (2 μg/ml) | 616 ± 24 | ND | ND | ||
| SHH plus PHCCC |
182 ± 3a
|
ND |
ND |
||
Values are means of 9-12 determinations from three to four independent experiments. p <0.05 (1-way ANOVA plus Fisher's PLSD) compared with the corresponding values obtained in the absence of PHCCC (a) or with values obtained with PHCCC alone (b). ND, Not determined.