Table 3.
Inhibition of [3H]thymidine incorporation by PHCCC in cultured cerebellar granule cells from wild-type and mGlu4-/- mice
|
|
[3H]thymidine incorporation (dpm/dish) × 10−3 |
||
|---|---|---|---|
|
|
Wild type |
mGIu4-/- |
|
| BME | |||
| Control | 260 ± 34 | 245 ± 9 | |
| PHCCC (10 μm) | 174 ± 7* (−33%) | 224 ± 16 (−8%) | |
| BME plus 10% FCS | |||
| Control | 336 ± 19 | 380 ± 35 | |
| PHCCC (10 μm) |
210 ± 12* (−37%) |
392 ± 15 (+3%) |
|
Cells from wild-type or knock-out mice were plated at the same density (1.5 × 106 cells per 35 mm culture dish), and cultures were exposed to PHCCC for 24 hr. Values are means ± SEM of six determinations from two independent experiments.
*
p < 0.05 (Student's t test) versus the respective controls.