Figure 8.

Grafted NG2⁺/EYFP⁺ cells differentiate into region-specific interneurons and mature oligodendrocytes in the OB. Tissue analysis was performed at 3 WAT. A, B, A significant percentage of grafted EYFP⁺ cells are still proliferative in the OB, as demonstrated by Ki67 staining (A3, B3; red). C-F, Grafted EYFP⁺ cells display mature OB interneuron markers. EYFP⁺ cells were stained with anti-NeuN (C5, D5, E5, F5; blue), anti-GAD-67 (D4; red), anti-Dlx (E4; red), and anti-Er81 (F4; red). Tissue was also processed for immunohistochemistry with anti-GFP (C4; red) to confirm the identity of graft-derived cells. G1-G5, Grafted EYFP⁺ cells were labeled with the oligodendrocyte marker O4 (G4; red). H1-H6, Grafted EYFP⁺ cells also displayed a mature oligodendrocyte phenotype, based on CNP protein expression (H4; red) and absence of GFAP (H5; blue). In C2, D2, E2, F2, G2, and H2, EYFP expression was color converted to black and white to show the full morphology of the grafted cells. The boxes in A1 and B1 are shown at a higher magnification in A2-A4 and B2-B4. Arrows indicate cells shown at higher magnification. Scale bars, 50 μm.