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. 2004 Feb 11;24(6):1358–1365. doi: 10.1523/JNEUROSCI.4022-03.2004

Figure 5.

Figure 5.

Immunohistochemistry for glial glutamate transporters. The expression of the glial glutamate transporters GLT-1 (A-C) and GLAST (D-F) is restricted to astrocytes with high expression levels of EGFP. A-C, Brainstem slices of P9 TgN(GFAP-EGFP) mice expressing EGFP in astrocytes (A) were stained for GLT-1 (B). As evident from the merged image (C), astrocytes with high EGFP fluorescence (arrows) show overlapping GLT-1 labeling and EGFP fluorescence, whereas astrocytes with lower EGFP expression (arrowheads) do not show any expression of GLT-1. D-F, EGFP (D) and GLAST (E) expression in astrocytes. Simultaneous expression (F) of EGFP and GLAST was found in brightly fluorescent astrocytes (arrow); staining for GLAST was not detectable in astrocytes with low EGFP expression (arrowheads). Images are single optical sections of confocal microscopy. Scale bars: (in C, F) A-F, 20 μm.