Effects of glutamate blockers and repetitive stimuli on the slow signal. A-C, Effects of APV and CNQX on the evoked optical signals. The data were obtained from E16 preparations with maxillary (V2) nerve stimulation. Thicker traces show controls, and thinner traces were obtained in APV (200 μm)-containing solution (A), CNQX (5 μm)-containing solution (B), and APV (190 μm)- and CNQX (5 μm)-containing solution (C). D, Decreasing slow signal amplitude with repetitive stimuli (0.1 Hz). The relative amplitudes of the slow signals in response to maxillary (V2) nerve stimulation are plotted against the time in seconds. Data were obtained from an E16 preparation. The different symbols correspond to different positions.