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. 2004 Feb 11;24(6):1398–1405. doi: 10.1523/JNEUROSCI.4260-03.2004

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Copyright © 2004 Society for Neuroscience 0270-6474/04/241398-08.00/0

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Figure 4. — Lack of postsynaptic GFP::βL accumulation in DIV8 HNs. A, Triple immunofluorescence of VIAAT (A1), GAD65 (A2), and GlyT2 (A3). Note lack of GlyT2 immunoreactivity. B, Example of an HN expressing endogenous GlyRs (mAb4a) that were partially colocalized with VIAAT-containing terminals. Inset in B shows the presence of mRNAs coding for GlyR α2 and β subunits. Top bands in each lane represent β-actin amplification products. C, Distribution of transfected GFP::βL. Note the diffuse cytosolic localization of GFP::βL and its absence opposite GAD65-labeled terminals (arrowheads). Scale bars, 10 μm.