Figure 2.

Sema4D induces clustering of Plexin-B1 in parallel with inactivation of R-Ras. A, COS-7 cells transfected with Myc- and HA-tagged full-length Plexin-B1 and GFP-tagged Rnd1 were stimulated with Sema4D for the indicated times. The cell lysates were immunoprecipitated (IP) with anti-Myc antibody. B, COS-7 cells transfected with Myc-Plexin-B1, GFP-Rnd1, and HA-R-Ras were stimulated with Sema4D for the indicated times. Then the cell lysates were incubated with GST-RBD, and bound R-Ras was detected with anti-HA antibody. The results shown are representative of five independent experiments that yielded similar results. FL, Full length.