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Antibody clustering of the cytoplasmic domain of Plexin-B1 stimulates the GTPase activity of R-Ras. Recombinant R-Ras preloaded with [γ-32P]GTP was incubated with the recombinant cytoplasmic domain of Plexin-B1 with (filled symbols, solid lines) or without (empty symbols, dashed lines) antibody-based clustering. The remaining radioactivity bound to R-Ras on the indicated times was measured by a nitrocellulose filtration assay. WT, Wild type.
