Figure 5.

Indirect IHC analysis of rod and cone presence in premetamorphic and postmetamorphic winter flounder retina. The source of reacted cryosections (premetamorphic or postmetamorphic retina) is indicated to the left of each row, and the identity of the screened primary antibody is indicated along the top of each column. Cones, as labeled by zpr-1, are evident in both premetamorphic and postmetamorphic retinas (left column). Rods, as labeled by either zpr-3 (middle column) or anti-rhodopsin (right column), are evident in postmetamorphic retina (both antibodies label an antigen located at the outer segments) but are absent from premetamorphic retina (arrows indicate the regions of expected labeling; i.e., the location of photoreceptor outer segments). The scale bar applies to all panels.