Table 1.

Primers and TaqMan probes used for real-time PCR

Primers and probes	Nucleotide sequences	Position	Product lengths
hGluR flip se	5′-TCAGTGAGSMAGGCGTCTTAGA	2300, 2321, 2354, 2324
hGluR flip as	5′-GTCTTGTCCTTACTTCCRGAGTC	2371, 2392, 2425, 2395
hGluR flip probe se	FAM-5′-AAGCTGAAAARCAAATGGTGG-TACGATAAAGG-TAMRA	2323, 2344, 2377, 2347	94 bp
hGluR flop se	5′-TTAACCTSGCAGTATTAAAACTGA	2279, 2300, 2333, 2303
hGluR flop as	5′-TGGARTCACCTCCCCCGCTG	2364, 2375, 2418, 2388
hGluR flop probe se	FAM-5′-TGGACAAATTGAAAAACAAATG-GTGGTACGA-TAMRA	2318, 2339, 2372, 2342	105 bp

Position 1 is the first nucleotide of the initiation codon. “Se” and “as” mark sense and antisense primers and probes. The TaqMan probes were labeled at 5′ with 6-carboxyfluorescein (FAM) and at 3′ with 6-carboxytetramethylrhodamine (TAMRA). All primers and probes were located on conserved nucleotides for each respective splice form. The antisense hGluR flip primer has one mismatch with hGluR2 and 4. The sense hGluR flop primer has one mismatch with hGluR2 and 4 and two with hGluR1.