Figure 7.

Conditioned medium from rhGAL-1/Ox-stimulated macrophages promotes Schwann cell migration from a transected site of a cultured DRG explant with an associated nerve stump. Pictures of migrating Schwann cells from a central transected-nerve site were taken after 4 d in culture. Numbers of Schwann cells migrating longer than 300 μm from the transected site are 22 in the control (A) and 86 in cultures treated with conditioned medium (B). As shown in Aa, the same region indicated by a white rectangular line in A, migrating cells were positive to immunohistochemical staining with antibody against S100. Scale bars: a, 20 μm; B, 100 μm. This effect of the conditioned medium was also seen in the central site after 6 d in culture. The Schwann cell numbers migrating longer than 500 μm in the conditioned medium (n = 10) is specifically larger than a control medium (n = 7) (p < 0.01).