Figure 3.

Behavioral effects of PRO in tg271Q-300 mice. A, PRO quiets tremor in hyperekplexic mice. Shown are typical 1 sec recordings obtained from wt and tg271Q-300 mice suspended by the tail from a mechanical transducer. In the upper traces, wt mice show no tremor activity before (solid line) and 3 min after (dotted line) a 15 mg/kg, i.p., injection of PRO. In the lower traces, untreated tg271Q-300 mice show high-frequency (25-30 Hz) tremors (solid line) that disappear 3 min after treatment with PRO. B, Acute pain responses to thermal stimuli in the hot-plate test. No significant differences in the latencies of withdrawal from thermal stimuli were observed between PRO-treated and control mice 3 and 8 min, after the intraperitoneal injection of 15 mg/kg PRO when using the hot-plate test (n = 11). C, PRO shortens the righting times of tg271-300 mice (white columns) without affecting those of wt mice (gray columns). Righting time latencies represent the time required for achieving a standing position either before (Control), or 3, 8, or 30 min after 15 mg/kg, i.p., injections of PRO. ^*p < 0.05 versus controls; ^**p < 0.01 versus controls (n = 11).