Figure 4.

V₁ agonist-induced suppression of IL-1β is dependent on CREB activation. A, Western blot data on V₁ agonist-induced CREB activation in cortical astrocytes. Each bar represents the mean ± SEM (n = 3 for each condition). ^*p < 0.05 versus control (CTRL). B, Western blot data on CREB level after CREB antisense, sense, and random oligonucleotide treatment for overnight. CREB AS was used to knock down CREB in the cortical astrocytes. S and R were used as control conditions to rule out nonspecific effects of CREB antisense. Each bar represents the mean ± SEM (n = 3 for each condition). ^*p < 0.05 versus control. C, Astrocytes were treated according to the indicated conditions for 24 hr followed by RNA extraction and RT-PCR on IL-1β. Each bar represents the mean ± SEM (n = 3 for each condition). ^*p < 0.05 versus control; ⁺p < 0.05 versus LPS-treated culture.