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. 2004 Mar 3;24(9):2304–2312. doi: 10.1523/JNEUROSCI.4162-03.2004

Figure 4.

Figure 4.

Tyrosine-phosphorylated human tau tyr18 is present in neuronal tau. A, SH-SY5Y cell lysate (left lane) and GST-fyn SH3 bound proteins from SH-SY5Y cells (right lane) were immunoblotted with anti-PY18. Arrow marks SH-SY5Y tau; asterisk protein was a cross-reacting species. B, SH-SY5Y cell lysate (lane 1) and 25, 100, 400, and 1000 ng of E. coli tau (lanes 2, 3, 4, and 5, respectively) were immunoblotted with monoclonal antibody 9G3. C, Mouse brain (30 μg of tissue lysate per lane) was probed with anti-PY18, 9G3, or a mixture of tau monoclonal antibodies 5E2 and tau5. Lysates were from (1) E18, (2) 1 d postnatal, (3) 3 d postnatal, (4) 1-week-old, (5) 2-week-old, (6) 3-week-old, (7) 1-month-old, and (8) 1-year-old mouse. Probing of lysates from 2-, 3-, and 6-month-old mice showed data similar to that of the 1-month-old mouse.