Figure 5.

Overexpression of NgR^WT causes axon retraction. GFP-labeled axons in the optic nerve proximal to the injury site 2 weeks after nerve surgery and lens injury. A, C, Growth-sensitized control transfected with AAV-GFP. B, D, Growth-sensitized case transfected with AAV-NgR^WT-IGFP. Note reduced number of axons proximal to the injury site (B, asterisk) and axons turning away from the long axis of the nerve (D, arrows). A, B, Arrowheads show the head of the myelinated portion of the nerve. In all cases, the lesion site (not shown) is to the right of the area depicted. Scale bar, 100 μm.