Figure 5.

Dynamics of the early adult outgrowth of ddaE. Single frames from a multiphoton time-lapse movie of a neuron expressing the CD8::GFP reporter are shown. Acetone was applied at 24 hr APF. Samples were acquired every 10 min starting at 39 hr APF. The thin processes are filipodia and are distributed along the length of the primary dendrites. Branches are born at interstitial positions where filopodia have remained stable for a number of frames and generate growth cones. Between E and F, a thin process tipped with a growth cone becomes engorged with CD8::GFP (arrowhead). Substantial branches are labile at this early stage and may persist for ≥ 1 hr before they are removed. The left branch in A is lost within 1 hr (arrow). Similarly, the branch labeled in G (arrow) is lost ∼3 hr later. Scale bar, 30 μm (also see movie 1, available at www.jneurosci.org).