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Local application of juvenile hormone mimic in wax. A, Projected confocal z-stack of ddaE expressing the CD8::GFP reporter imaged at 72 hr APF. Wax containing JHm was applied at 24 hr APF to the lateral region of the pupal cuticle overlying half of abdominal tergite 4 (see diagram). B, Projected confocal z-stack of ddaE from the opposite side of the pupa at 72 hr APF. Arrowheads denote ddaE. Scale bar, 45 μm.
