Figure 9.

Dynamics of the early adult outgrowth of ddaE after JHm treatment at 0 hr APF. Single frames from a multiphoton time-lapse movie of ddaE expressing the CD8::GFP reporter are shown. JHm was applied at 0 hr APF. Samples were acquired every 10 min for 5 hr starting at 39 hr APF. The 85 hr APF image shows the simple arbor that is generated from such a treatment. The thin processes are filipodia (arrowheads). The protrusions on the growth cone (arrows) are attempts to generate branches, but all are retracted during the 5 hr movie (also see movie 3, available at www.jneurosci.org). Scale bar, 15 μm.