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. 2019 Aug 30;10:747. doi: 10.3389/fgene.2019.00747

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Copyright © 2019 Dahlin, Holkenbrink, Marella, Wang, Liebal, Lieven, Weber, McCloskey, Wang, Ebert, Herrgård, Blank and Borodina

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Strain growth in minimal media and nitrogen-depleted media; Growth curves of S. cerevisiae (A, C) and Y. lipolytica strains (B, D) on minimal media (A, B) and nitrogen-depletion media (C, D). Pre-cultures were grown on minimal media until the late exponential phase, and subsequently washed and resuspended in either fresh minimal media or in nitrogen-depletion media. Data shown are mean values ± standard deviations of biological triplicates. Sc, S accharomyces c erevisiae; Yl, Y arrowia l ipolytica, Pr, fatty alcohol producing; Np, non-producing; Gr, growth phase; Ni, nitrogen-depleted stationary phase.