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. 2019 Aug 30;13:398. doi: 10.3389/fncel.2019.00398

FIGURE 1.

FIGURE 1

gp120 does not cause neurite pruning in p75NTR– /– neurons. Representative images of primary neuronal cultures prepared from E17 cortex of WT or p75NTR– /– mice. At 7 days in culture, neurons were exposed to heat inactivated (boiled) gp120IIIB (control) or 5 nM gp120IIIB for 6 h. Cells were fixed and processes visualized by anti TUBB3 antibody (green). DAPI (blue) was used to stain nuclei. Bar = 20 μm. Note that the neuronal network (TUBB3 positive processes) in p75NTR– /– neurons exposed to gp120 is preserved when compared to WT neurons exposed to gp120. The experiment was repeated twice.