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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2019 Sep;50(1):e96. doi: 10.1002/cpsc.96

Figure 4.

Figure 4.

Comparison of CTB differentiation from StemPro- vs. E8-adapted WA09/H9 hESCs, using BMP4+IWP2.

A) First-step differentiation of the StemPro- and E8-adapted H9s do not show any morphological differences.

B) Flow cytometric analysis at the end of the first step (day 4) did not show a difference in EGFR expression.

C) qPCR for lineage-specific markers. The pluripotency marker, POU5F1, is decreased and CTB markers (p63, CDX2 and GCM1) are increased by day 4 following BMP4+IWP2 treatment, with no difference between the StemPro- and E8-adapted cells. Data are normalized to 18S and expressed as fold-change above StemPro-day 0 (undifferentiated state).