| Component | Amount of stock for
final volume of 50 ml |
Stock
concentration |
Final concentration |
|---|---|---|---|
| KO DMEM | 24.5 ml | -- | -- |
| F12 | 24.5 ml | -- | -- |
| ITS | 500 μl | 100X | 1X |
| L ascorbic acid | 96 μl | 33.3 mg/mL | 64 μg/mL |
| NaHCO3 | 362 μl | 75 mg/mL | 543 μg/mL |
| BSA | 3.3 ml | 30% | 2% |
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