| Component | Amount of stock for
final volume of 50 ml |
Stock
concentration |
Final concentration |
|---|---|---|---|
| DMEM/F12 | 39 ml | -- | -- |
| NEAA | 500 μl | 100X | 1X |
| Glutamax | 500 μl | 100X | 1X |
| Knockout Serum Replacement | 10 ml | 100% | 20% |
| 2-Mercaptoethanol | 91 μl | 55mM | 0.1 mM |
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