Figure 2.

SMYD3 was efficiently suppressed in bladder cancer cells. Bladder cancer cell line T24 was infected with three shRNA-SMYD3 plasmids. (A) Bright field and image and fluorescence images of the GFP reporter were captured by a fluorescent microscope (magnification, ×100). mRNA level was measured by qRT-PCR. (B) Densitometric analysis and Western blot analysis compared with β-actin. Statistical analysis was calculated with unpaired Student’s t-test, n≥3.

Abbreviations: shRNA, short hairpin RNA; GFP, green fluorescence protein.