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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Breast Cancer Res Treat. 2019 Jun 22;177(2):345–355. doi: 10.1007/s10549-019-05324-7

Fig. 1.

Fig. 1

TRPC1/4 expression correlates to TNBC cell line sensitivity to EA. a TRPC1 b TRPC4 and c TRPC5 expression was determined by qRT-PCR analysis of MDA-MB-231, HCC1806, BT-549, and Hs578T cells. Fold difference was analyzed as compared to MDA-MB-231 expression levels. Significance determined by one-way ANOVA with Dunnett’s multiple comparisons test *p = 0.02, ****p < 0.0001 d The effects of EA on BT-549, Hs578T, MDA-MB-231, and HCC1806 cell survival were measured by the SRB assay after 48 hr treatment (n=3–15). The effects of reduced TRPC1 and TRPC4 expression on EA mediated cytotoxicity were assessed by SRB assay following 48 hr siRNA-mediated knockdown of TRPC1 and TRPC4 and 24 hr EA treatment in e BT-549 and g Hs578T cells (n=3). The efficiency of TRPC1 and TRPC4 knockdown was determined by qRT-PCR analysis of f BT-549 and h Hs578T cells compared to the expression in mock transfected cells (n=3)