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. Author manuscript; available in PMC: 2019 Nov 6.
Published in final edited form as: Nat Metab. 2019 May 6;1(5):560–569. doi: 10.1038/s42255-019-0066-3

Fig. 1. ABHD5 and PNPLA3 co-traffic to endoplasmic reticulum.

Fig. 1.

COS-7 cells on 25 mm coverslips were co-transfected with pairs of ABHD5-mCherry and EYFP; mCherry and PNPLA3-EYFP; or ABHD5-mCherry with PNPLA3-EYFP and loaded with OA (200 μM) overnight. Cells were fixed with 4% paraformaldehyde and imaged on a Zeiss LSM 800 laser scanning microscope equipped with an Airyscan detector. mCherry was pseudocoloured red and EYFP pseudocoloured green in merged images to show co-localization (yellow). Results are from one experiment and are representative of four independent experiments. Scale bar denotes 10 μm.