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. Author manuscript; available in PMC: 2019 Nov 6.
Published in final edited form as: Nat Metab. 2019 May 6;1(5):560–569. doi: 10.1038/s42255-019-0066-3

Fig. 3. The interaction between ABHD5 and PNPLA3 is regulated by endogenous and synthetic ligands of ABHD5.

Fig. 3.

(a) Gluc protein complementation (PC) assay of HEK293A cells transfected with GlucN-ABHD5 and either PNPLA3-GlucC or PNPLA2-GlucC and treated with BSA or 200 μM oleic acid (OA) the following day for three hours. Data are biological quadruplicates from four independent experiments. ***p=0.0001 effect of OA as determined by two-way ANOVA with Bonferroni post t-test. (b) HEK293A cells expressing GlucN-ABHD5 and PNPLA3-GlucC were treated with either BSA, 200 μM OA, or 200 μM oleic acid ethyl ester (OAEE) for 30 minutes. Data are biological quadruplicates from three independent experiments. ***p=0.0001 indicates a significant difference between BSA and OA as determined by one-way ANOVA with Bonferroni post t-test. (c) Gluc PC assay was performed in HEK293A cells as in a, except cells were treated for one hour with SR-3420 (20 μM). Data are biological quadruplicates from five independent experiments. ***p=0.0001 indicates a significant effect of SR-3420 as determined by two-way ANOVA with Bonferroni post t-test. Data are expressed as means +/− SEM. ns, not significant.