Fig 9.

(a) Recombinant Arabian camel IFNε alters the morphology of breast cancer cell lines MDA-MB-231 (upper) and MCF-7 (lower). (b) Interferon epsilon inhibits the survival of breast cancer cells. Cells were treated with different concentrations of IFNƐ for 48 h. MTT assay was performed and percentage cell viability was calculated compared to control cells. GraphPad Prism 6 was used to calculate the IC₅₀ of IFNƐ: 5.65±0.2 μM and 3.91±0.6 μM for MDA-MB-231 and MCF-7 cells respectively. Experiments were repeated at least 3 times in triplicate. (c) Interferon epsilon induces apoptosis in breast cancer cells. Cells were treated with 5 μM IFNε protein for 48 h. Apoptosis assay was performed, and the percentage cell viability was calculated (*p<0.5, **p<0.1 and ***p<0.01). (d) Expression of caspase-3 in MDA-MB-231 cell line untreated and recombinant IFNε treated cells at a concentration of 3 and 6 μM.