Fig 2. Confocal laser scanning microscopy analysis of the membrane integrity of S. Typhimurium (ATCC 14028) treated with methyl gallate (MG), tylosin (Ty) and various concentrations of their combination (MT).
SYTO9/PI staining was applied to differentiate cells with intact membranes (green) from those with damaged membranes (red). Salmonella: non-treated S. Typhimurium. MG = 128 μg/mL; Ty = 256 μg/mL; ¼× MT = 32 μg/mL MG and 64 μg/mL Ty; ½× MT = 64 μg/mL MG and 128 μg/mL Ty; and 1× MT = 128 μg/mL MG and 256 μg/mL Ty.