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. 2019 Sep 6;14(9):e0221952. doi: 10.1371/journal.pone.0221952

Fig 4. FTH1 knockdown lowers cell viability of T3691 but not T387 GICs following radiation.

Fig 4

GICs were transfected with FTH1 or control siRNA at siRNA (μg): liposome (μl) ratios of 2:4 (T3691) or 2:8 (T387) for 24h, followed by no radiation (0Gy) or radiation at 4Gy and 8Gy. Cell viability was assessed 24 and 48 hours after radiation exposure using MTS assay. (A, B) In T3691 GICs, at both 24h (a) and 48h (b) post radiation, cell viability in FTH1 siRNA treated group was significantly reduced relative to control siRNA irrespective of radiation at 0Gy, 4Gy and 8Gy (*** = p<0.001). FTH1 siRNA treated T3691 cells radiated at 8Gy showed an additive decrease in cell viability relative to un-irradiated controls (0Gy) (Λ = p<0.01) as well as cells radiated at 4Gy (Λ # = p<0.05). However, there was no significant reduction in viability at 4Gy relative to un-irradiated controls (0Gy). (C) 24h post radiation, T387 GICs also showed significant decrease in cell viability at all three doses (*** = p<0.001). (D) T387 GICs showed no decrease in cell viability at 48h post radiation (ns = not significant). T387 GICs showed no significant change in cell viability after radiation exposure relative to un-irradiated controls (ns = not significant). Data from three experiments was normalized to liposome treated controls.