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Characterization of the binding of Glmn and the FK1 domain of FKBP51* (MonoCys mutant covalently labelled with Fluorescein-5-Maleimide = 5-MF) via a homogeneous time-resolved fluorescence resonance energy transfer (HTRF) assay. Molecules that bind to the binding sites on FKBPs or Glmn can be quantified in a competitive setup. HIS = polyhistidine-tag, GST = glutathione S-transferase tag.
