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. 2019 Sep 6;14(9):e0221926. doi: 10.1371/journal.pone.0221926

Fig 2. Development of an HTRF-based assay for binding of FKBPs and Glmn.

Fig 2

(A) Fluorescein-labelled FKBP51FK1 and FKBP12 bind to the TAMRA-labelled FKBP ligand FK[4.3.1]-16g as observed by a destructive FRET. Corresponding KD values are indicated below and represent mean values and standard deviation of three independent dilution series; (B) Titration of GST-Glmn to 30nM Fluorescein-labelled protein in the presence of 0.8 nM Anti GST-Tb cryptate followed by HTRF readout (Ex: 485 nm, Em: 520 / 620 nm).