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. 2019 Sep 6;14(9):e0221926. doi: 10.1371/journal.pone.0221926

Fig 5. Characterization of FKBP51 active site mutants.

Fig 5

(A) FKBP51FK1 (1–140) and mutants F67V bind to the high-affinity TAMRA-labelled tracer FK[4.3.1]-16g with similar affinity in the fluorescence binding assay, while binding of the mutant FD67/68DF is compromised. (B) FKBP51 mutants F67V bind with slightly and FD67/68DV with substantially reduced affinity to GST-Glmn in the competitive HTRF assay. KD or IC50 values are indicated below and represent mean values and standard deviation of three independent dilution series.