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. 2019 Aug 26;15(8):e1008010. doi: 10.1371/journal.ppat.1008010

Fig 2. Deletion of gltA leads to diminished metabolic flexibility and distinct amino acid auxotrophy.

Fig 2

(A) Heatmap summarizing BioLog Phenotype Microarray analysis of WT KPPR1 and KPPR1ΔgltA growth in 282 carbon and nitrogen limited growth conditions indicates multiple conditions that sustained growth of WT KPPR1, but not KPPR1ΔgltA. (B) A subset of growth conditions summarizing glycolysis and non-essential amino acid biosynthesis that indicates a distinct amino acid auxotrophy is induced by deletion of gltA. Arrows from citric acid cycle intermediates indicate amino acids that utilize these intermediates for biosynthesis. (C) A subset of growth conditions summarizing dipeptide utilization that further indicates induction of a distinct amino acid auxotrophy by deletion of gltA (n = 3, mean displayed, *P < 0.05, Student’s t test). Underlined substrates support equivalent or enhanced growth of KPPR1ΔgltA relative to WT KPPR1.