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. 2019 Aug 26;13(8):e0007704. doi: 10.1371/journal.pntd.0007704

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© 2019 Gent et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Production of IL-4 cytokine in supernatants during Specific (HPV, SEA and SWAP) and non-specific stimulation (CONA) and absence of stimulant (MED) in Schisto-infected+HPV, Schisto/PZQ+HPV vaccine and HPV-only (Control) groups. PBMCs collected from the 3 groups (n = 3) at the 4 time points; Week 0 (pre-vaccination), Week 4 (4 weeks after the 1^st vaccination and before the booster), Week 6 (6 weeks after the 1^st vaccination and 2 weeks after the 2^nd dose), Week 8 (8 weeks after 1^st dose and 4 weeks after 2^nd dose). These PBMCs were stimulated with respective Antigens (HPV, SEA, SWAP and CONA) for 48h, after which the supernatants were analyzed using ELISA. The bars represent the mean IL-4 levels of each group at each time point. Non-parametric analysis (Kruskal Wallis, Dunn’s post hoc analysis) was applied. Data was considered statistically significant if p≤0.05.