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. 2019 Aug 26;13(8):e0007660. doi: 10.1371/journal.pntd.0007660

Fig 1. AAS27 is highly transcribed at adult unfed stage.

Fig 1

(A-C) AAS27 relative expression analysis in unfed (UN), 24, 48, 72, 96, and 120 h fed adult female tick dissected tissues: salivary glands (SG), midguts (MG) and remnants as carcass (CA) analyzed by qRT-PCR. In three biological replicates, relative expression levels of AAS27 were analyzed using the 2−ΔΔCt method using ribosomal protein S4 (RPS4) transcript as internal reference. The lowest expressed time point was used as a calibrator for each tissue. (D-G) AAS27 qualitative expression analysis were performed by western blot in in unfed (UN), 24, 72, and 120h fed tick dissected salivary glands (SG), midguts (MG), synganglion (SYN), Malpighian tubules (MT), ovary (OV) and remnants as carcass (CA), eggs 22 days after oviposition (E), unfed larvae (L), unfed nymphs (N), unfed adult females (F) and males (M). Protein extracts were subjected to western blotting analyses using a monospecific antibody (purified) against yeast-expressed rAAS27 (0.30 μg/μL in a 1:500 dilution). rAASrAAS-27 (500 ng) was used as positive control (C+).