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. 2019 Aug 26;13(8):e0007660. doi: 10.1371/journal.pntd.0007660

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© 2019 Tirloni et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — Purified rAAS27 was treated with deglycosylation enzyme mix (D+) or without treatment (D-) and resolved on a 12% SDS-PAGE following western blotting analysis with: (A) rabbit serum generated by repeated A. americanum adult tick infestation (dilution 1:50 and 1:250), (B) rabbit serum generated by repeated adult I. scapularis infestations (dilution 1:50), (C) rabbit serum generated by nymph I. scapularis infestations (dilution 1:50), (D) monospecific antibody produced against yeast-expressed rAAS27 was used as positive control (0.30 μg/μL in a 1:2,000 dilution) and (E) and rabbit pre-immune serum (dilution 1:50).