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Release of NGAL by neutrophils during degranulation is unchanged by prior uptake of NPs. Neutrophils previously incubated with nanoparticles for 2 h at 1 μg/mL (PS) or 5 μg/mL (liposomes) exhibit no change in their ability to release NGAL, a critical degranulation marker, upon stimulation with 1 μM fMLP, 50 nM PMA, or 1 μM ionomycin for 30 min at 37 °C. n = 2. * = p < 0.05.
