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. 2019 Sep 6;8:e49188. doi: 10.7554/eLife.49188

Figure 2. Role of intracellular domains in voltage-dependent gating.

Figure 2.

(A) Voltage family current trace of WT Eag1, Eag1TM, and the Eag1/hErg chimera with the voltage-pulse protocol shown above. (B) Normalized tail current vs. depolarization voltage plot for WT Eag1 (black square, n = 6), Eag1TM (green circle, n = 4), and the Eag1/hErg chimera (red triangle, n = 4) with V0.5 values (mean ± sd). Eag1TM did not reach saturation up to 100 mV. (C) Cole-Moore effect of WT Eag1, Eag1TM, and the Eag1/hErg chimera with the voltage-pulse protocol shown above. (D) Plot of normalized current at 10 ms following the depolarization step vs holding potential for WT Eag1 (Cole-Moore I-V plot). The Cole-Moore I-V plot was fit with a Boltzmann function to estimate the holding potential that produces half maximal rates of activation (V0.5CM = −126 ± 0.9 mV, mean ± sd, n = 6).