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. Author manuscript; available in PMC: 2020 Sep 5.
Published in final edited form as: Cell. 2019 Aug 22;178(6):1362–1374.e16. doi: 10.1016/j.cell.2019.07.014

Figure 4. WaTx is a mode-specific gating modifier.

Figure 4

(A-C) Inside-out HEK cell patch recordings (Vh = −40 mV) of spontaneous, WaTx (100 nM) or AITC (10 μM)-evoked TRPA1 openings, quantified with respect to (B), dwell time (n = 6–8 patches/treatment) and (C) latency to first opening (n = 13–14 patches/treatment). One-Way ANOVA with post-hoc Holm-Sidak correction for multiple comparisons.

(D) Inside-out HEK cell patch recordings (Vh = −40 mV) showing WaTx (1 μM) effect on a single, irreversibly activated (Iodoacetamide, 100 µM) TRPA1 channel ( n = 9–11 patches/treatment), quantified with respect to (E) dwell time. Unpaired two-tailed Student’s t-test.

(F) TRPA1 ionic selectivity and conductance for spontaneous, WaTx (100 nM)- or AITC (50 μM)-evoked openings (n = 3–7 HEK cell patches/condition). Two-way ANOVA with post-hoc Holm-Sidak correction.

All summary data, mean ± SEM. See also Figure S4.