Representative biological aptamers. (A) DARPin scaffold, with constant N- and C-terminal modules, and 3 copies of the ankyrin repeat module, with the sequence of that module shown below. The library is built through substitutions at the residues indicated by the red ‘x’. (B) The Adhiron affimer scaffold, which was derived from a large number of plant cystatin sequences. The library is built through substitutions at the purple-colored loops, which are expanded beyond the 7 indicated residues. The figures in A and B are from Tamaskovic, R. et al., Methods …
2012, 503, 101 (ref. 172) and Tiede, C. et al., Elife
2017, 6, e24903 (ref. 44), respectively).