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. 2019 Jun 5;27(9):1621–1637. doi: 10.1016/j.ymthe.2019.05.023

Figure 2.

Figure 2

The Positive Correlation between PTB-AS and PTBP1 Expression and PTB-AS Regulates the PTBP1 mRNA and Protein Levels

(A and B) Expression of PTB-AS and PTBP1 mRNA in the (A) glioma cell lines (n = 12) compared to human normal astrocyte lines (n = 4) or (B) glioma tissues (n = 83) compared to normal brain tissues (n = 6) detected by quantitative real-time PCR. Expression data are presented as the mean ± SD, n = 3. *p < 0.05, **p < 0.01 (Student’s t test). (C) Correlation analysis showing that PTB-AS correlated positively with PTBP1 mRNA or protein expression. The mRNA and protein expression levels were measured in the glioma tissue samples. The delta cycle threshold (dCt) values (normalized to GAPDH) were subjected to Pearson correlation analysis (Student’s t test). (D and E) Changes in PTB-AS expression altered PTBP1 mRNA and protein levels. T98G cells and U87MG cells were infected with (D) two different siRNAs (PTB-AS si1 or PTB-AS si2) to knock down (siNC [small interfering negative control]) or (E) PTB-AS-plenti6 plasmid to overexpress PTB-AS (EGFP-plenti6 was the negative control). PTB-AS and PTBP1 mRNA expression were measured by quantitative real-time PCR. PTBP1 protein level was measured by western blot analysis, n = 3. *p < 0.05, **p < 0.01 (Student’s t test).